Journal: Cell Proliferation

Article Title: RNAi‐mediated inhibition of the desmosomal cadherin (desmoglein 3) impairs epithelial cell proliferation

doi: 10.1111/j.1365-2184.2011.00765.x

Figure Lengend Snippet: Modulation of Dsg3 expression affected cell proliferation in MDCK cells. (a) Western blotting of cell lysates extracted from cells with either overexpression (knock‐in: KI) or knockdown (KD) of Dsg3. (b) Confocal microscopy of MDCK cells with ectopic Dsg3 expression [myc‐tag in (i)] or with Dsg3 knockdown (iii). Images in (ii) were vector control cells. (c) Dispase fragmentation assay of MDCK cells with either vector or Dsg3 shRNAi transduction. Cells were grown to confluence before being treated with 2.4 units/ml dispase for about 30 min to detach epithelial sheets. These sheets were subjected to mechanical stress by pipetting five times with 1 ml tips. Epithelial fragments were quantified by ImageJ and increased fragments (up to 2‐fold) were seen in cells with Dsg3 silencing by shRNAi‐1 or shRNAi‐2, respectively. Data are averages of duplicates in each group. (d) Growth curve of matched MDCK cells with up‐ or down‐regulation of Dsg3. Cells with overexpression had higher proliferation, but those with Dsg3 knockdown exhibited lower growth rate compared to matched control cells.

Article Snippet: HuSH TM shRNA plasmids (29‐mer) used for transduction in MDCK cells were purchased from OriGene Technologies Inc (Rockville, MD, USA).

Techniques: Expressing, Western Blot, Over Expression, Knock-In, Confocal Microscopy, Plasmid Preparation, Transduction

Journal: Nucleic Acids Research

Article Title: A supramolecular assembly formed by influenza A virus genomic RNA segments

doi: 10.1093/nar/gkr985

Figure Lengend Snippet: Effect of silent mutations S71 and R73 on viral replication and on incorporation of vRNA 7. ( A ) MDCK cells were infected at a m.o.i. of 10 −4 with wild-type recombinant A/Moscow/10/99 (H 3 N 2 ) virus or a virus bearing silent mutations at M2 codons 71 and 73. The release of viral progeny into the supernatant was monitored by determining the tissue culture infective dose (TCID50). Points correspond to the mean of two experiments; the data ranges are smaller than the symbol size. ( B ) Strategy and output of the competition experiment.

Article Snippet: MDCK cells were purchased from Cambrex Bioscience (ATCC, CCL34), Walkersville, MD, USA.

Techniques: Infection, Recombinant, Virus